Simulation instruction

1. Click on "Start the Experiment" button
2. Check "0.1 M NaOH" checkbox and click on the button "Fill Burette".
3. Select an amino acid from the dropdown. Next, click on "Show Conical Flask" button.
4. Check "Show pH meter" checkbox to measure pH of amino acid solution.
5. Click on the the pH meter image to immerse it in the conical flask for pH measurement. Click on the pH meter image to remove it from the conical flask.
6. Click on the conical flask to position it beneath the burette.
7. Next click on the "Titrate" button. Click on the burette's stopcock to initiate titration. After initiation, click on the conical flask to mix NaOH with the amino acid. Terminate the titration by clicking on the burette's stopcock.
8. Click on the conical flask to place it under the pH meter and measure the pH value.
9. Repeat instruction number 5 to 8.
10. Click on "Data Analysis" button.

pI calculation instruction

1. Check "Table to get pK_a1,pK_a2,and pK_R values of amino acid" checkbox.
2. Calculate and enter the pI value in the input box. Consider pK_a1 and pK_a2 values of chosen amino acid from the table . Next click on "Check" button to display a message indicating correctness. To reveal the correct answer, click on the "Show Answer" button.
3. For basic amino acids consider pK_a2 and pK_R values of chosen amino acid from the table. Calculate and enter pI value in the input box. Next click on "Check" button to display a message indicating correctness. To reveal the correct answer, click on the "Show Answer" button.
4. For acidic amino acids consider pK_a1 and pK_R values of chosen amino acid from the table. Calculate and enter pI value in the input box. Next click on "Check" button to display a message indicating correctness. To reveal the correct answer, click on the "Show Answer" button.

Note: To repeat the experiment, click on the "Re-Do the Experiment" button.

Start the Experiment Re-Do the Experiment
Step 1	The pH of the amino acid solution is lowered using 0.1 M HCl. Then, 0.1 M NaOH is used for titration of the chosen amino acid to determine its isoelectric point.
Step 2	0.1 M NaOH Fill Burette
Step 3	Select an Amino Acid Glycine (G) Alanine (A) Valine (V) Leucine (L) Isoleucine (I) Serine (S) Threonine (T) Cysteine (C) Methionine (M) Proline (P) Phenylalanine (F) Tyrosine (Y) Tryptophan (W) Histidine (H) Asparagine (N) Glutamine (Q) Glutamic acid (E) Aspartic acid (D) Lysine (K) Arginine (R) Show Conical Flask Show pH meter
Step 4	Titrate
Step 5	Data Analysis Data Analysis Data Analysis
Step 6	Table to get pKa1,pKa2, and pKR values of amino acid

Check Show Answer

For basic amino acids Check Show Answer

For acidic amino acids Check Show Answer

1. Enter net charge value in the given input box. Click the "check" button to indicate the correctness. To reveal the correct answer, click on the "Show Answer" button.
2. To calculate pI of the given peptide, enter the value in the given input box. Click the "check" button to indicate the correctness. To reveal the correct answer, click on the "Show Answer" button.

Check Show Answer