Virtual Labs
IIT Kharagpur
Experimental Biochemistry Virtual Laboratory
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List of Experiments
Computation of various physical and chemical properties of Proteins and DNA from their primary sequence
DNA amplification by polymerase chain reaction (PCR)
DNA analysis by agarose gel electrophoresis
pH-titration of amino acids and small peptides, and estimation of isoelectric point (pI)
Protein analysis by polyacrylamide gel electrophoresis (PAGE)
Protein purification by affinity chromatography
Protein purification by size exclusion chromatography (SEC)
Protein-DNA interaction analysis by electrophoretic mobility shift assay (EMSA)
Thermodynamic characterization of protein-ligand binding by isothermal titration calorimetry (ITC)
Kinetic characterization of protein-ligand binding by surface plasmon resonance (SPR)
