DNA analysis by agarose gel electrophoresis


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DNA analysis by agarose gel electrophoresis

Introduction

Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight on an agarose gel. The smaller-sized fragments migrate faster than larger ones; the distance migrated on the gel varies inversely with the logarithm of the molecular weight. In this experiment, we have three main objectives listed as follows:

  1. Preparation of an agarose gel
  2. Running an agarose gel
  3. Visualization of DNA