Next click on checkboxes of step 1 and click "Annealing fluorescently-labelled DNA
probe" button.
Select all the component for preparing 1 x binding buffer and click the button "1 x
Binding Buffer Done" in
step 2
Now, select the required components from the dropdown for mixing protein sample.
Now to prepare diluted sample, click on the dropdown and consider all the sample (from A
to E).
Next, click on "Incubate in ice bucket" button.
Click on "Tris-borate-ETDA buffer" button to load in the electophoresis chamber.
Now, Click on the "Sample Loading" dropdown and select "Load Sample A". Click on the
pipette to draw the sample into the pipette. Once the pipette is place over the first
well, click on the pipette to load the sample. Repeat for sample B, C, D
and E.
Next click on "Setting up Electrophoresis" button. Click on the top cover of the
electrophoresis to close it.
Now to connect, click on the negative connector of electrophorersis
chamber, drag to negative connector of power supply. Next click on the
positive connector of electrophorersis chamber and drag to positive connector of power
supply. For any
wrong connection, click on the wire to disconnect. (The positive connector is colored
red, whereas the negative connector is colored black.)
Now click on "V" sign of the power supply to switch ON. And click on "+" sign of the
power supply to set the voltage between 100 V and 120 V.
Click on the run icon button to run the gel. The dye front will reaches the bottom of
the gel in dark conditions. Click on stop icon button to switch OFF the power and
disconnect the wires (click on the wire to disconnect).
Click on the top cover of the electrophoresis to open it.
Click on "Visualize the protein-DNA" button to visualize under Fluorescein channel.
Note: To repeat the experiment, click on the "Re-Do the Experiment" button.
